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Articles

Novel Antibodies for the Simple and Efficient Enrichment of Native O-GlcNAc Modified Peptides

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By Rajan Burt, Borislav Dejanovic, Kimberly A. Lee, Hayley J. Peckham
| mcponline.org
Highlights • Anti-O-GlcNAc antibodies are fast and simple enrichment reagents. • Anti-O-GlcNAc antibodies are sensitive and achieve significant depth of coverage. • Anti-O-GlcNAc antibodies are specific for singular O-GlcNAc modifications. • Anti-O-GlcNAc antibody enrichment techniques can be applied to cells and tissues. • HCD product-triggered EThcD data acquisition improves depth of coverage.
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