Kimberly Morgan

Abstract Thousands of nuclear pore complexes (NPCs) cover the nuclear surface of mammalian cells and establish selective transport conduits that biochemically segregate the nucleoplasm and cytoplasm. Although the molecular composition and structure of archetypical NPCs are well understood, distinct NPCs composed of varying nucleoporins exist in different cell types and even within individual cells. Furthermore, the integration of NPCs within mechanosensitive networks impacts their dilation state.

By Kimberly Morgan and Suzanne Thornsbury, University of Florida The COVID-19 pandemic exposed weaknesses in food supply chains, prompting renewed interest in innovation. Technologies like artificial intelligence (AI), precision farming, and robotics offer potential solutions, but their success depends on how well they are integrated into real-world farming systems. Mechanization is not a one-size-fits-all solution.

Abstract The exploration of cell-type and environmentally-responsive nuclear pore complex (NPC) plasticity requires new, accessible tools. Using pan-Expansion Microscopy (pan-ExM), NPCs were identified by machine learning-facilitated segmentation with resolved cytoplasmic rings (CR), inner rings (IR) and nuclear rings (NR). They exhibited a large range of diameters with a bias for dilated NPCs at the basal nuclear surface in clusters suggestive of local islands of nuclear envelope (NE) tension.