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Klim Kolyvanov

As seen in: BioRxiv
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Articles

Analysis of developmental gene expression using smFISH and in silico staging of C. elegans embryos

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By Laura Breimann, Ella Bahry, Klim Kolyvanov, Marwan Zouinkhi
| BioRxiv
Abstract Regulation of transcription during embryogenesis is key to development and differentiation. To study transcript expression throughout Caenorhabditis elegans embryogenesis at single-molecule resolution, we developed a high-throughput single-molecule fluorescence in situ hybridization (smFISH) method that relies on computational methods to developmentally stage embryos and quantify individual mRNA molecules in single embryos.
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RS-FISH: Precise, interactive and scalable smFISH spot detection using Radial Symmetry

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By Ella Bahry, Laura Breimann, Leo Epstein, Klim Kolyvanov
| BioRxiv
RS-FISH: Precise, interactive and scalable smFISH spot detection using Radial Symmetry Ella Bahry, Laura Breimann, Leo Epstein, Klim Kolyvanov, Kyle Harrington, Timothee Lionnet, Stephan Preibisch bioRxiv 2021.03.09.434205; doi: https://doi.org/10.1101/2021.03.09.434205
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