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Kurt W Loedige on Muck Rack

Kurt W Loedige

As seen in: BioRxiv
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Harnessing RNA-based DNA repair pathways for targeted gene editing

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By Nhan Huynh, Sin Kwon, Thomas A McMurrough, Kurt W Loedige
| BioRxiv
Abstract Recent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that the asymmetric DNA overhangs generated by the small TevSaCas9 dual nuclease informs a simple and robust RNA-based editing strategy in human cells whereby Polθ and Rad52 are recruited to repair the double-strand break.
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