Lara Wischer

Discussion Here, we mapped spatial immunotypes of HCC that predict for outcome from immunotherapy by applying deep spatial single-cell level profiling and bioinformatic deconvolution of the immune architecture of the HCC TME. Three major patterns of immune cell infiltration were observed that were defined by differing amounts and distributions of infiltrating CD8 T-cells between the tumour parenchyma and stroma: an immune-depleted, a compartmentalised and an immune-enriched immunotype.

Methods For IMC analysis, formalin-fixed, paraffin-embedded (FFPE) samples from n=54 HCC patients were obtained after surgery as a discovery cohort at the Institute of Pathology of the University Hospital Heidelberg, Germany (online supplemental table 2).