Lars Mathias Blank

Abstract The plasic crisis is ominipresent, from littering macroplastic to reports that document plastic in every niche of this planet, including the human body. In order to achieve higher recycling quotas, especially of mixed plastic waste, pyrolysis seems to be a viable option. However, depending on the process parameters, plastic pyrolysis oil waste is encountered, which is difficult to valorize, due to the enormous spread of the molecules included.

Abstract Pseudomonas putida has become an increasingly important chassis for the production of valuable bioproducts. This development is not at least due to the ever-improving genetic toolbox, including gene and genome editing techniques. Here, we present a novel, one plasmid design of a key genetic tool, the pEMG/pSW system, guaranteeing one engineering cycle to be finalized in three days.