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The HPLC-FLD method was validated according to conventional requirements. As shown in Fig.1, the retention times of venlafaxine, demethylcitalopram, and citalopram were 3.8, 7.2, and 8.1 min, respectively. The analytes were well separated with no interference. The linear regression curve for demethylcitalopram was established using the ratio of peak area to concentration.
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