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Leonid Gubar

As seen in: Science Magazine, BioRxiv
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Articles

CRISPR-Cas9 HDR Optimization: RAD52, Denatured and 5'-Modified DNA Templates in Knock-In Mice Generation

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By Boris V. Skryabin, Daniela Braun Verified, Helena Kaiser, Leonid Gubar
| BioRxiv
Abstract CRISPR/Cas9-mediated genome editing is a powerful tool for producing animal models of human diseases. However, it often encounters challenges related to low efficiency of donor DNA templates insertion through homology-directed repair (HDR) pathway or unwanted insertions and/or multiplications. Here, we present findings from multiple targeting experiments aimed at generating a Nup93 conditional knockout (cKO) mouse model.
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Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events

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By Boris V. Skryabin, Delf-Magnus Kummerfeld, Leonid Gubar, Birte Seeger
| Science Magazine Verified
Abstract CRISPR-Cas9–mediated homology-directed DNA repair is the method of choice for precise gene editing in a wide range of model organisms, including mouse and human. Broad use by the biomedical community refined the method, making it more efficient and sequence specific. Nevertheless, the rapidly evolving technique still contains pitfalls.
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Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events

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By Boris V. Skryabin, Delf-Magnus Kummerfeld, Leonid Gubar, Birte Seeger
| Science Magazine Verified
Research ArticleGENETICS Boris V. Skryabin1,*, Delf-Magnus Kummerfeld1, Leonid Gubar1, Birte Seeger1, Helena Kaiser1, Anja Stegemann1, Johannes Roth2, Sven G. Meuth3, Hermann Pavenstädt4, Joanna Sherwood5, Thomas Pap5, Roland Wedlich-Söldner6, Cord Sunderkötter7, Yuri B. Schwartz8, Juergen Brosius9,10 and Timofey S. Rozhdestvensky1,* 1Medical Faculty, Core Facility Transgenic Animal and Genetic Engineering Models (TRAM), University of Muenster, Muenster, Germany.
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