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Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging
1. Introduction For the 300,000 patients who are diagnosed with invasive breast cancer or carcinoma in situ each year in the United States, the primary treatment method is lumpectomy (breast-conserving surgery) or mastectomy (breast-removal surgery).
A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues Adam K Glaser, Kevin W Bishop, Lindsey A Barner, Robert B Serafin, Jonathan T.C. Liu bioRxiv 2020.05.06.081745; doi: https://doi.org/10.1101/2020.05.06.081745
Solid immersion meniscus lens (SIMlens) for open-top light-sheet microscopy
Abstract Open-top light-sheet (OTLS) microscopy has been developed for rapid volumetric imaging of large pathology specimens. A challenge with OTLS microscopy is the transmission of oblique illumination and detection beams through a horizontal sample plate without introducing aberrations. Previous solutions prevented vertical sample movement, constrained the refractive index of the sample, and/or hindered multi-resolution imaging.
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