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Lisaweta Roßmannek on Muck Rack

Lisaweta Roßmannek

As seen in: Nature
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Articles

Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation

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By Antonios D. Konitsiotis, Lisaweta Roßmannek, Angel Stanoev, Malte Schmick
| Nature Verified
In order to compare the localisations of polybasic-stretch-containing Src and dually palmitoylated Fyn, both SFKs were C-terminally fused to mCitrine (Src-mCit and Fyn-mCit) and expressed in HeLa cells. These recombinant proteins preserved the regulation of their autocatalytic activity and were expressed at approximately equal or lower levels as compared to endogenous SFKs (Supplementary Fig. 1).
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