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Liyin Du on Muck Rack

Liyin Du

As seen in: MDPI
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A Real-Time Recombinase Polymerase Amplification Assay for Specific Detection of Lumpy Skin Disease Virus

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By Qi Zhai, Xia Zhou, Nan Yang, Liyin Du
| MDPI
3. Results 3.1. Primer and Probe Selection Through the multiple sequences alignment and analysis, the selected forward primer sequence (LSD-F) has a difference of 5 bases from the GTPV AV41 vaccine strain, including a 4-nt deletion (TTTT). The reverse primer (LSD-R) and the probe (LSD-Pro) sequence have differences of 3 bases and 7 bases from the vaccine strain, respectively (Figure 1).
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