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Lovelesh Vashist

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A general strategy towards multicolour fluorogenic peptides for wash-free bioassays

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By Lorena Mendive-Tapia, Utsa Karmakar, Zandile Nare, Kohei Iijima, Kazuya Kikuchi, Xiaogang Liu, Marc Vendrell, Lovelesh Vashist, Karolina Tokarczyk
| Nature Verified
Abstract Peptide-based fluorescent probes are valuable tools for live-cell imaging, but conventional labelling approaches often require washing or long incubation, limiting their signal-to-noise ratios. Fluorogenic amino acids (FgAAs) can overcome these limitations, yet their rational design remains challenging owing to the lack of generalizable quenching principles and synthetic routes compatible with diverse fluorophores.
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