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Lucinda Barrett on Muck Rack

Lucinda Barrett

As seen in: Environment and Behavior, BioRxiv, medRxiv, Microbiology Research
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Articles

Comparison of direct cDNA and PCR-cDNA Nanopore sequencing of RNA from Escherichia coli isolates

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By Gillian Rodger, Samuel Lipworth, Lucinda Barrett, Sarah Oakley
| Microbiology Research
Article metrics loading... /content/journal/mgen/10.1099/mgen.0.001296 Download as PowerPoint /deliver/fulltext/mgen/10/10/mgen001296.html?itemId=/content/journal/mgen/10.1099/mgen.0.001296&mimeType=html&fmt=ahah References Bayega A, Oikonomopoulos S, Wang YC, Ragoussis J. Improved nanopore full-length cDNA sequencing by PCR-suppression. Front Genet 2022; 13:1031355 [View Article] [PubMed] [Google Scholar] Stark R, Grzelak M, Hadfield J. RNA sequencing: the teenage years.
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Estimating the effect of antimicrobial resistance genes on minimum inhibitory concentration in Escherichia coli

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By Samuel Lipworth, Kevin Chau, Sarah Oakley, Lucinda Barrett
| medRxiv
The authors have declared no competing interest. The computational aspects of this research were funded from the NIHR Oxford BRC with additional support from the Wellcome Trust Core Award Grant Number 203141/Z/16/Z. SL was funded by an MRC Clinical Research Training Fellowship MR/T001151/1. ASW and TEAP are also supported by the NIHR Oxford Biomedical Research Centre. ASW is an NIHR Senior Investigator. NS is an NIHR Oxford BRC Senior Fellow.
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Comparison of direct cDNA and PCR-cDNA Nanopore sequencing of Escherichia coli isolates

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By Gillian Rodger, Samuel Lipworth, Lucinda Barrett, Sarah Oakley
| BioRxiv
Abstract Whole-transcriptome (long-read) RNA sequencing (Oxford Nanopore Technologies, ONT) holds promise for agnostic analysis of differential gene expression (DGE) in pathogenic bacteria, including for antimicrobial resistance genes (ARGs). However, direct cDNA ONT sequencing requires large concentrations of polyadenylated mRNA, and amplification protocols may introduce technical bias.
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