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Lukram Sushil Singh on Muck Rack

Lukram Sushil Singh

As seen in: Nature
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Design and transfection of CRISPR/Cas9 constructs for the myostatin gene in Labeo rohita muscle cells

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By Gowhar Iqbal, Nevil Pinto, Arvind Sonwane, Kiran D. Rasal, Mukunda Goswami, Darshan Pawaskar, Lukram Sushil Singh, Nidarshan N. Chikkathimmashetty
| Nature Verified
Abstract Myostatin (mstn) is a negative regulator of skeletal muscle growth and is considered as an important target for enhancing aquaculture production. The present study aimed to design and validate single-guide RNAs (sgRNAs) and CRISPR/Cas9 constructs for exon 1 of the mstnb gene in Labeo rohita, and to evaluate their transfection efficiency in the L. rohita dorsal muscle (LRDM) cell line at the 10th, 20th, and 30th passages.
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