Mahlon Collins

Abstract In genotype-by-environment interactions (GxE), the effect of a genetic variant on a trait depends on the environment. GxE influences numerous organismal traits across eukaryotic life. However, we have a limited understanding of how GxE shapes the molecular processes that give rise to organismal traits. Here, we characterized how GxE shapes protein degradation, an essential molecular process that influences numerous aspects of cellular and organismal physiology.

Flow cytometry. We characterized our proteasome activity TFTs using flow cytometry. For all flow cytometry experiments, we inoculated yeast strains into 400 μL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30°C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day, 4 μL of each saturated culture was inoculated into a fresh 400 μL of G418-containing SC -lys media and cells were grown for an additional 3 hours prior to flow cytometry.