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Ca2+ translocation We performed SH imaging of a variety of lipid GUV membranes that have the same PC and PA headgroups in a 1:1 mol ratio but different alkyl chains: DOPC:DOPA, DOPC:DOPA:Chol, DPhPC:DPhPA, and SLPC:SLPA. GUVs from these different lipids were imaged repeatedly (period t = 1 min and accumulation time 5 s) after adding 5 mM CaCl2 into the outside solution. Fig.
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