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Marco Barchi

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Articles

Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes - Nature Communications

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By Ihsan Dereli, Michiel Boekhout, Marc Gentzel, Marco Barchi, Arp Schnittger, John Weir, Franz Herzog, Hasibe Tuncay, Teresa Giannattasio
| Nature Verified
Abstract Programmed DNA double-strand break (DSB) formation is a crucial feature of meiosis in most organisms. DSBs initiate recombination-mediated linking of homologous chromosomes, which enables correct chromosome segregation in meiosis. DSBs are generated on chromosome axes by heterooligomeric focal clusters of DSB-factors. Whereas DNA-driven protein condensation is thought to assemble the DSB-machinery, its targeting to chromosome axes is poorly understood.
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Extending resolution within a single imaging frame - Nature Communications

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By Esley Torres-García, Damián Martínez Verified, Damián Martínez, Eduardo Brito-Alarcón, Héctor H. Torres-Martínez, Joseph G. Dubrovsky, Christopher Wood, Alvaro H. Crevenna, Raúl Pinto-Cámara, Carlos Calcines-Cruz, Haydee O. Hernández, Marco Barchi, Rocco D’Antuono, Diego Krapf
| Nature Verified
Abstract The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light.
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