MINFLUX is a recent single-molecule localization microscopy technique. The technique allows nanoscale resolution imaging and fast tracking. A number of biological problems can be addressed with this technique. 1 Introduction: From Resolution to Precise Localization The importance of high resolution and sensitivity in optical microscopy, especially aiming at advances in life sciences applications, is well known and recognized (Diaspro 2010; Diaspro & van Zandvoort, 2021).