Margo Diricks

MATERIALS AND METHODS The beta-lactamase-negative reference strain H. influenzae Rd KW20 and evolved mutant strains were routinely grown from −80°C freezer stocks on chocolate agar (Mueller–Hinton broth with 5 g/L yeast extract, 17 g/L agar, 5% defibrinated horse blood, and 20 mg/L beta-nicotinamide adenine dinucleotide [beta-NAD]) at 37°C in a humid atmosphere containing 5% CO2.

Abstract The evolution of antimicrobial resistant (AMR) pathogens poses a global health threat and it is unclear if evolutionary trajectories to resistance lead to predictable phenotypes. We analyzed AMR-evolution in the human pathogen Haemophilus influenzae in controlled evolution experiments with increasing concentrations of either ampicillin, cefotaxime, or ceftriaxone.

Margo Diricks ORCID: orcid.org/0000-0002-5428-14561,2, Thomas A. Kohl1,2, Nadja Käding3,4, 3, Susanne Hauswaldt3, Omar Jiménez Vázquez1, Christian Utpatel1,2, Stefan Niemann1,2 na1, Jan Rupp3,4 na1 & Matthias Merker1,2,5 na1 Genome Medicine volume 14, Article number: 13 (2022) Cite this article 20 Accesses 2 Altmetric Metrics details Abstract Background Bacteria belonging to the genus Haemophilus cause a wide range of diseases in humans. Recently, H.