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Blood oxygen regulation via P2Y12R expressed in the carotid body - Respiratory Research
All procedures involving animal care and use in this study were performed using wild-type (C57/Bl6N) and P2ry12 gene deficient (P2ry12–/–) or littermate control (P2ry12+/+) mice of mixed gender, aged 8–14 weeks, with an average weight of 30 ± 4.0 g. The original breeding pairs of P2ry12–/– knockout mice, B6;129-P2ry12tm1Dgen/H were obtained from Deltagen Inc. (San Matteo, CA, USA). Cloning and breeding strategy, and genotyping protocol have been described previously [23].
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