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Maria Barthmes on Muck Rack

Maria Barthmes

As seen in: Wiley Online Library, MDPI
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Articles

Resolving three GABA induced electrogenic events and GABA‐coupling stoichiometry for Na+ and Cl− in hGAT‐1

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By Rocco Zerlotti, Maria Barthmes, Kim Cole
| Wiley Online Library Verified
A activating (external) buffer NA non-activating (external) buffer R resting (internal) buffer SSME solid-supported membrane-based electrophysiology Introduction The secondary active gamma-aminobutyric acid (GABA) : sodium symporter GAT-1 is part of the solute carrier 6 (SLC6) family. GAT-1 is expressed in presynaptic neurons and glial cells, where it is the most abundant GABA transporter among all GAT proteins [[1, 2]].
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IJMS | Free Full-Text | A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screen

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By Andre Bazzone, Cecilia George, Maria Barthmes, Nina Brinkwirth
| MDPI
4. Materials and Methods 4.1. Cell Culture A human embryonic kidney (HEK293) cell line expressing TMEM175 under control of a tetracycline inducible expression system was used for the purpose of this project. As controls in the respective experiments, HEK293 UT cell lines were used for APC recordings, and a parental HEK293 cell line was employed for SSME recordings.
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