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Next-Generation Sequencing in a Direct Model of HIV Infection Reveals Important Parallels to and Differences from In Vivo Reservoir Dynamics

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By Marilia Rita Pinzone, Maria Paola Bertuccio, Morphofunctional Imaging, D. Jake VanBelzen
| asm.org
Pathogenesis and Immunity Marilia Rita Pinzone, Maria Paola Bertuccio, D. Jake VanBelzen, Ryan Zurakowski, Una O’Doherty Guido Silvestri, Editor DOI: 10.1128/JVI.01900-19 ABSTRACT Next-generation sequencing (NGS) represents a powerful tool to unravel the genetic make-up of the HIV reservoir, but limited data exist on its use in vitro. Moreover, most NGS studies do not separate integrated from unintegrated DNA, even though selection pressures on these two forms should be distinct.
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