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Marjahan Akhtar

As seen in: Emerging Infectious Diseases, Nature, PLOS, MDPI, BioRxiv, The Lancet
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Articles

Evolution of pandemic cholera at its global source

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By Amber Lee Barton, Mokibul Hassan Afrad, Alyce Taylor-Brown, Nisha Singh, Chetan Thakur, Taufiqul Islam, Marjahan Akhtar, Taufiqur Bhuiyan, Ashraful Islam Khan, Neelam Taneja, Nicholas Thomson, Firdausi Qadri
| Nature Verified
Abstract The seventh pandemic of cholera, caused by the seventh pandemic El Tor lineage of Vibrio cholerae, was previously shown to have emanated in three global waves from the Bay of Bengal, bordering Bangladesh and India1. However, the respective roles of the Ganges Delta and Basin regions in seeding these global pandemic waves were not known. Here we show that, although transmission events occur between Bangladesh and India, V.
Open in Who Shared Issue with article?

Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

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By Zachary T. Berndsen, Scripps Research, Marjahan Akhtar, PLOS Pathogens
| PLOS
Sample preparation. Recombinant EtpA glycoprotein was exchanged to water using Microcon Ultracel PL-10 centrifugal filter. Glycoprotein was reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP-HCl) and alkylated with 10 mM 2-Chloroacetamide in 100 mM ammonium acetate for 20 min at room temperature (RT, 24°C). Glycoprotein was digested with 1:25 Proteinase K (PK) for 30 min at 37°C or 1:20 trypsin for 16 h at 37°C.
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Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

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By Marjahan Akhtar, Mahima Thapa, Aaron J. Schmitz, Jonathan Torres
| BioRxiv
Abstract Enterotoxigenic Escherichia coli (ETEC) cause hundreds of millions of cases of infectious diarrhea annually, predominantly in children from low-middle income regions. Notably, in children, as well as human volunteers challenged with ETEC, diarrheal severity is significantly increased severity in blood group A (bgA) individuals.
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