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We begin by examining representative results from employing this protocol for 96-well lifespan assays. In the first example, the method was used to screen through 30,000 chemicals to successfully identify chemicals that extend the lifespan of C. elegans, and also includes results from the follow-up re-test screen of the best performing chemicals using the same protocol. These results were previously described1.
We chose 22 Caenorhabditis natural isolates spanning three species (C. briggsae, C. elegans and C. tropicalis) to maximize sampling of genetic and geographic diversity within each species27,28. In terms of total divergence, the genetic differences encompassed among these strains is comparable to sampling genomes from mice to humans27.
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