Maya Kasowski

ArticleOnline now102548Open access 1Department of Genetics, Stanford University School of Medicine, 240 Pasteur Drive, Palo Alto, CA 94304, USA 2Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA 3Department of Biomedical Data Science, Stanford University School of Medicine, 1265 Welch Road, Stanford, CA 94305, USA 4Department of Pediatrics, Stanford University School of Medicine, 725 Welch Road,...

Abstract Oral immunotherapy (OIT) is the only U.S. Food and Drud Administration-approved treatment for peanut allergy. Peanut-reactive (pr) CD4+ T cells are pivotal in peanut allergy pathogenesis and OIT-induced desensitization. However, the underlying pr CD4+ T cell immune mechanisms leading to sustained unresponsiveness after OIT discontinuation are largely unknown.

For detailed methods on cell culture conditions and processing, see our previous study [19]. Briefly, 2×103 cells from each LCL were collected and pooled by population after growth to 6–8×105 cells/mL. To prevent disproportionate cell line growth within pools throughout the collection and pooling process, sub-pools were frozen in liquid nitrogen at −180 °C.