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Expanding the pH‐Shift Technique for Sequential Extraction of Intact Proteins and Sulfated Polysaccharide From Fish Heads: A Novel Biorefinery Approach
1 Introduction Recently, changes in lifestyle and growing awareness of seafood's nutritional benefits have led to an increase in global seafood consumption. This shift is evident from global seafood production exceeding 223.2 million tons in 2024, with aquaculture contributing 130.9 million tons (FAO 2022). Rainbow trout (Oncorhynchus mykiss ), a major aquaculture species, accounted for nearly 740,000 tons globally, with Iran producing over 212,000 tons (FAO 2022).
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