Meimei Yin

eLife assessment This important study uses single-cell RNA-seq to obtain a more granular understanding of cell subsets within allergic contact dermatitis in a model system with DNFB. The convincing data revela unique subpopulations of dermal fibroblasts as key responders to interferon gamma and likely as mediators of dermatitis. This study has many novel aspects and provides a unique resource as well.

1 INTRODUCTION Skin fibrosis is characterised by fibroblast proliferation, excessive extracellular matrix (ECM) deposition and the loss of specialised epithelial structures, which can cause debilitating and pathological changes in the skin, such as keloids, hypertrophic scars and scleroderma.1-5 Aberrant fibroblast activation during wound repair is a primary cause of skin fibrosis.4, 6, 7 However, fibroblasts are highly heterogeneous and plastic, and the origin of fibrotic fibroblast subtypes...

Abstract Allergic contact dermatitis (ACD), a prevalent inflammatory skin disease, is elicited upon repeated skin contact with protein-reactive chemicals through a complex and poorly characterized cellular network between immune cells and skin resident cells. Here, single-cell transcriptomic analysis of the murine hapten-elicited model of ACD reveals that upon elicitation of ACD, infiltrated CD4+ or CD8+ lymphocytes were primarily the IFNγ-producing type 1 central memory phenotype.