Mengying Zeng

Abstract Current single-cell atlases of mouse embryos are limited in temporal resolution and cellular or sequencing depth coverage. Here we report the mouse developmental Cell and Lineage Atlas (mdCLA), which covers 37 time points across the entire embryonic development from the 2-cell stage (E1.5) to birth (E19.0), with two million cells and a median of 4,500 genes detected per cell.

1Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), Changde, China 2Hengyang Medical College, University of South China, Hengyang, China Background: Transfer RNA-derived small RNAs (tsRNAs) have recently emerged as critical regulators in cancer biology; yet their expression profiles and clinical relevance in multiple myeloma (MM) remain poorly defined.

4. Materials and Methods 4.1. Animals All experimental procedures were conducted in accordance with the guidelines and approval of the Peking Union Medical College Hospital Institutional Animal Care and Use Committee, as evidenced by the approval letter Code (XHDW-2021-034). Adult male C57B/L6J mice (8 weeks old, weighing 20–22 g) were procured from the Chinese Academy of Medical Sciences.