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Reference Composition Biases Automated Cell Annotation and Obscures Biological Signals in Xenotransplantation Single-cell Atlases

Oct 02, 2026 |

By Lisha Mou, Ying Lu, MetaLife Lab

| Wiley Online Library

1 Introduction Clinical kidney xenotransplantation is entering a new era, with genetically engineered pig kidneys now transplanted into human recipients and non-human primates (NHPs), generating unprecedented opportunities to study cross-species graft biology [1-7].

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Reference Composition Biases Automated Cell Annotation and Obscures Biological Signals in Xenotransplantation Single-cell Atlases

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