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Biochemical characterization of Bombyx mori Dicer‐2 that dices double‐stranded RNAs into 20‐nt small RNA
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest. Supporting Information REFERENCES , , & (2007) Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. Insect Biochemistry and Molecular Biology, 37, 176–183. , , , & (2020) Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica. Current Research in Virological Science, 1, 100001. , , , , , et al.
Long DCL4-substrate dsRNAs efficiently induce RNA interference in plant cells
Abstract RNA interference (RNAi) is induced by the direct transfer of double-stranded RNAs (dsRNAs) into protoplasts prepared from Arabidopsis thaliana seedlings. In this protoplast RNAi system, we compared the efficacies of various-sized dsRNAs (between 21 and 139 nucleotides [nt]) for inducing RNAi and assessed the dsRNA-cleaving activities of Dicer-like 3 (DCL3) and 4 (DCL4).
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