Mikkel Boas Thygesen

1 Introduction Methods for protein conjugation are crucial as they enable the incorporation of small molecule reporters to study protein dynamics [1], create protein-based probes for biological investigations [2, 3], enhance industrial biocatalysts [4], and pharmaceutical biologics [5-7]. The ideal conjugation method fulfills a series of criteria: It uses mild conditions and provides high yields of homogeneous and stable products, where new functionalities are installed site-selectively.