Miles K. Huseyin

Abstract Micro-C and Region Capture Micro-C (RCMC) are developments of chromosome conformation capture (3C) genomics methods used to identify regions of the genome in close 3D proximity at nucleosome resolution. Micro-C uses in situ MNase digestion of crosslinked chromatin, biotin labeling, proximity ligation and biotin pulldown to recover ligated nucleosomal DNA fragments, with RCMC including a subsequent capture step to enrich for specific regions of the genome.

Abstract Although enhancers are central regulators of mammalian gene expression, the mechanisms underlying enhancer–promoter (E-P) interactions remain unclear. Chromosome conformation capture (3C) methods effectively capture large-scale three-dimensional (3D) genome structure but struggle to achieve the depth necessary to resolve fine-scale E-P interactions.

Abstract Polycomb repressive complex 1 (PRC1) is an essential chromatin-based repressor of gene transcription. How PRC1 engages with chromatin to identify its target genes and achieve gene repression remains poorly defined, representing a major hurdle to our understanding of Polycomb system function. Here, we use genome engineering and single particle tracking to dissect how PRC1 binds to chromatin in live mouse embryonic stem cells.