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Mirle van Geest

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Articles

Quantitative relationship between cycle threshold values and infectious influenza virus in patient nasopharyngeal samples

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By Jeroen van Kampen, Theo M. Bestebroer, Dennis de Meulder, Richard Molenkamp, Sander Herfst, Pieter Fraaij, Kain S. Saygan, Daphne G. J. C. Mulders, Mirle van Geest, Ron A. M. Fouchier
| Nature Verified
Abstract Real-time reverse transcription polymerase chain reaction (rRT-PCR) is nowadays commonly used to diagnose viral infections. This assay provides cycle threshold (Ct) values, which inversely correlate with viral load but do not directly indicate infectivity. The relationship between Ct values and infectivity remains unclear. As a first step, we assessed this relationship in nasopharyngeal samples from 97 seasonal influenza A (IAV) and 95 influenza B (IBV) virus-rRT-PCR-positive patients.
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