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Articles

A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila

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By Benedikt Rauscher, Florian Heigwer, Jun L Zhou, Amy Hess, Katharina Kern, Laura Lange, Bojana Pavlovic, Kristin S. Rädecke, Michael Boutros, Claudia Strein, Mona Stricker, Celine Beyersdörffer, Jana Frei, Nora Langner, Roberta Malamud, Lukas Schmitt, Lukas Voos, Erica Valentini
| eLife
Abstract Genetic screens are powerful tools for the functional annotation of genomes. In the context of multicellular organisms, interrogation of gene function is greatly facilitated by methods that allow spatial and temporal control of gene abrogation. Here, we describe a large-scale transgenic short guide (sg) RNA library for efficient CRISPR-based disruption of specific target genes in a constitutive or conditional manner.
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