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Seawater samples were collected from the mesopelagic layer of the western Pacific Ocean (130°04′42″E, 12°30′01″N) at a depth of approximately 300 m using a Niskin bottle. Approximately 1 L of seawater was aseptically collected, transported to the laboratory on ice, and processed within 24 h. For bacterial isolation, 1 mL of seawater was subjected to tenfold serial dilution (10−1–10−6) using sterile artificial seawater.
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