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Abstract CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition.
To examine the effects of maternal ageing on the meiotic apparatus, we obtained oocytes from naturally cyding women in two age groups, including younger (aged 20–25 years) and older (aged 40–45 years) women. Using high- resolution confocal microscopy we obtained a detailed picture of the meiotic spindle and chromosome placement during various phases of meiosls.
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