Naidi Sun

Abstract Microvascular function and oxygen metabolism are central to tissue and organ health. However, label-free methods for imaging oxygen dynamics in three-dimensional (3D) microvascular networks at the level of single red blood cells (RBCs)—the fundamental units of oxygen transport in vivo—remain lacking. Here, we introduce super-resolution functional photoacoustic microscopy (SR-fPAM), which spatiotemporally tracks RBC movements under dual-wavelength excitation.

eLife Assessment This is an important study that utilized in vivo optical measurements of the cortical metabolic rate of O2 and blood flow, as well as measurements in isolated mitochondria to assess the uncoupling of the oxidative phosphorylation due to hypoxia-ischemia injury of the neonatal brain, and effects of the hypothermia treatment.

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