Nakajohn Thewasano

eLife assessment This important study reports the identification of a new amino acid sequence motif (i.e., "internal beta-signal") on outer membrane proteins, which is recognized by beta-assembly machinery in gram-negative bacteria. The authors carried out rigorous experiments, providing compelling evidence in support of their conclusions. This work significantly advances our understanding of the biogenesis of outer membrane proteins.

New Results doi: https://doi.org/10.1101/2021.10.29.466387 Abstract Outer membrane proteins (OMPs) are essential components of the outer membrane of Gram-negative bacteria. In terms of protein targeting and assembly, the current dogma holds that a "β-signal" imprinted in the final β-strand of the OMP engages the β-barrel assembly machinery (BAM complex) to initiate membrane insertion and assembly of the OMP into the outer membrane.