Nam Ki Lee

Contrasting effects of mismatch locations on Z-DNA formation under bending force Z-DNA is a non-canonical, left-handed helical structure that plays crucial roles in various cellular processes. DNA mismatches, which involve the incorporation of incorrect Watson-Crick base pairs, are present in all living organisms and should contribute to the mechanism of Z-DNA formation. However, the impact of mismatches on Z-DNA formation remains poorly understood.

Special Issue Published as part of The Journal of Physical Chemistry B virtual special issue “Advances in Cellular Biophysics”. Fluorescent proteins (FPs) have become indispensable tools in molecular and cellular biology, serving as unparalleled tools for the real-time visualization and tracking of dynamic biological processes.

Abstract Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene locus to the nucleoid periphery correlates with transcription, driven by either E. coli RNAP or T7 RNAP, and the effect is potentiated by translation.