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Nathan Barrabes

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Articles

Diversity-generating retroelements for programmable targeted hypermutagenesis

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By Paul Rochette, Elena López-Rodríguez, David Wen, Léo Régnier, Catherine Fan, Anna Maikova, William Rostain, Linhan Wang, Nicolas Wolff, Rémi Monasson, Simona Cocco, Seth L. Shipman, Raphael Laurenceau, David Bikard, Imran Nooraddin, Paul Vittot, Nathan Barrabes, Karol Melissa Cerdas-Mejías, Auguste Bouvier, Thea Chrysostomou, Orso Subrini
| Nature Verified
Abstract Diversity-generating retroelements (DGRs) are natural systems that accelerate the evolution of diverse bacterial functions through targeted hypermutation. We establish a method using DGRs coupled to recombineering (DGRec), which enables the diversification of any sequence of interest in Escherichia coli. Detailed characterization of reverse transcriptase sequence biases demonstrates how it maximizes the exploration of the sequence space while avoiding nonsense mutations.
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