Navid Rabiee

ACS Publications —

ACS Publications- JACS Au — The rapidly evolving field of live-cell super-resolution imaging has transformed our understanding of cellular structures and dynamic biological processes. This perspective delves into the importance and challenges of multicolor super-resolution volume imaging in the context of living cells, where the ability to visualize multiple molecular species simultaneously across three dimensions is critical for deciphering complex cellular functions. While recent innovations have made significant strides, challenges such as temporal and spatial resolution limits, photobleaching, and depth of field remain significant obstacles. This work explores emerging strategies aimed at overcoming these technical barriers, including the development of novel fluorophores, advanced computational techniques leveraging artificial intelligence, and hardware innovations in imaging systems. By addressing these challenges, the field is poised to move toward a future where high-precision, multicolor live-cell volume imaging becomes routine, enabling real-time visualization of intricate molecular interactions. The conclusion emphasizes that we are on the brink of a new frontier in cellular imaging, one that promises to revolutionize biological research and disease treatment by providing unprecedented access to the molecular mechanisms governing life at its most fundamental level.

ScienceDirect — Two-dimensional (2D) materials have emerged as transformative building blocks in bioelectronics, enabling seamless interfaces between electronic systems and living tissues. Their exceptional electrical conductivity, mechanical flexibility, optical tunability, and environmental resilience make them uniquely suited for next-generation biomedical devices. This perspective highlights recent advances in the use of 2D materials for bioelectronic applications, including skin-conformal wearables and implantable interfaces for dynamic organs such as the heart, brain, and gastrointestinal tract. We examine cutting-edge fabrication techniques, including transfer printing, layer-by-layer assembly, and hybrid integration with three-dimensional architectures, with an emphasis on scalability and clinical translation. The manuscript also addresses critical challenges—ranging from long-term biocompatibility and environmental stability to regulatory hurdles—and presents current solutions including the design of biodegradable platforms and multilayered heterostructures with multimodal capabilities. By bridging materials science, bioengineering, and medicine, 2D materials hold the promise of revolutionizing healthcare through smart, minimally invasive, and adaptive bioelectronic systems capable of real-time monitoring, diagnosis, and therapeutic intervention.

ACS Publications — The development of precise and efficient delivery systems is pivotal for advancing CRISPR/Cas9 gene-editing technologies, particularly for therapeutic applications. Engineered metal–organic frameworks (MOFs) have emerged as a promising class of inorganic nonviral vectors, offering unique advantages such as tunable porosity, high cargo-loading capacity, and biocompatibility. This review explores the design and application of MOF-based nanoplatforms tailored for the targeted delivery of CRISPR/Cas9 components, aiming to enhance gene-editing precision and efficiency. By incorporating stimuli-responsive linkers and bioactive ligands, these MOFs enable controlled release of CRISPR/Cas9 payloads at the target site. Comparative discussions demonstrate superior performance of MOFs over conventional nonviral systems in terms of stability, transfection efficiency, and reduced off-target effects. Additionally, the intracellular trafficking mechanisms and the therapeutic potential of these platforms in preclinical models are discussed. These findings highlight the transformative potential of MOF-based delivery systems in overcoming the challenges associated with gene-editing technologies, such as immunogenicity and cytotoxicity, paving the way for their application in precision medicine. This review provides a blueprint for the integration of nanotechnology and genome editing, advancing the frontier of nonviral therapeutic delivery systems.

Nature — Chronic care manages long-term, progressive conditions, while acute care addresses short-term conditions. Chronic conditions increasingly strain health systems, which are often unprepared for these demands. This study examines the burden of conditions requiring acute versus chronic care, including sequelae. Conditions and sequelae from the Global Burden of Diseases Study 2019 were classified into acute or chronic care categories.

Taylor & Francis — Introduction The advent of 3D printing has revolutionized biomedical engineering, yet limitations in creating dynamic human tissues remain. The emergence of 4D printing, which introduces time as a fourth dimension, offers new possibilities by enabling the production of adaptable, stimuli-responsive structures. A thorough literature search was performed across various databases, including Google Scholar, PubMed, Scopus, and Web of Science, to identify pertinent studies published up to 2025. The search parameters were confined to articles published in English that concentrated on peer-reviewed clinical studies Areas covered This review explores the transition from 3D to 4D printing and focuses on stimuli-responsive materials, particularly hydrogels, which react to environmental changes. The literature search examined recent studies on the interaction of these materials with biological stimuli, emphasizing their application in tissue engineering and drug delivery applications. Expert opinion 4D printing, combined with smart materials, holds immense promise for advancing biomedical treatments, including customized therapies and regenerative medicine. However, technological challenges must be addressed to realize its full potential.

Springer — Lung cancer is one of the most deadly types of cancer, worldwide. It is essential to diagnose lung cancer in the early stage to accelerate the therapeutic process. The progress in nanotechnology-assisted therapies has revolutionized early diagnosis of lung cancer by developing non-invasive, selective, and highly sensitive nanostructures.

Springer — Lung cancer is the leading cause of cancer-related deaths, worldwide. To date, various strategies have been developed and examined for treating lung cancer. The era of nanotechnology has opened a new avenue for designing advanced nanoparticulate systems for single or multiple delivery of chemotherapeutics. Despite the promising synergism between these systems, some challenges still remain.

ScienceDirect — This paper presents a comparative perspective on the use of hydrogen-bonded organic frameworks (HOFs) and metal-organic frameworks (MOFs) in the development of biosensors. Both HOFs and MOFs have gained significant attention due to their tunable structures, high surface areas, and potential for a wide range of applications, including environmental monitoring, medical diagnostics, and biosensing. HOFs, with their non-metallic nature and reliance on hydrogen bonds, offer unique advantages such as easier synthesis, biocompatibility, and enhanced stability in certain environments. In contrast, MOFs, which incorporate metal centers, exhibit superior mechanical strength, higher stability, and versatility in sensing a broader range of analytes. This paper reviews the key features, advantages, and limitations of HOFs and MOFs in the context of biosensor development. It highlights recent advancements in their applications for detecting biological targets, and exploring their sensitivity, selectivity, and response times. Additionally, the paper discusses the challenges in integrating these materials into functional biosensors and provides insights into their future potential for improving diagnostic tools, environmental monitoring, and healthcare applications. Also, the paper offers a critical evaluation of how HOFs and MOFs can complement each other in advancing biosensor technology.

ScienceDirect — In battling global pathogens like SARS-CoV-2, salmonella, and malaria, rapid and precise detection methods are vital. Traditional approaches often face cost and accessibility challenges, especially in resource-limited areas. Integrating CRISPR-Cas technology into detection processes is a game-changer, offering unparalleled specificity and speed for point-of-care use. This method aligns with Green Biomaterials principles, emphasizing eco-friendliness and resource efficiency. By incorporating green biomaterials, the CRISPR-Cas system becomes more sustainable, efficient, and environmentally sensitive. This integration revolutionizes pathogen detection while promoting environmental stewardship. It not only advances rapid detection but also underscores a commitment to ecological consciousness and sustainability in diagnostics, offering promise for global health.

Wiley Online Library — Multiview super-resolution microscopy (MSRM) has revolutionized biological imaging by providing unprecedented spatial resolution, enabling detailed visualization of complex biological structures and processes. This review explores recent advancements in MSRM, including its integration with deep learning, enhanced imaging dimensionality, synchronized multiview capture, and optimized deconvolution techniques. Despite these significant strides, the field continues to face challenges such as intricate implementation protocols, extensive data management requirements, and the need for precise calibration. Future progress is likely to focus on automating data acquisition workflows, integrating MSRM with complementary imaging modalities for enriched analysis, and achieving real-time imaging capabilities to capture dynamic biological processes with greater accuracy. Furthermore, MSRM holds immense potential in biomedical research, offering transformative insights into disease mechanisms and the interactions between materials and biological systems. By overcoming existing limitations and embracing emerging technologies, MSRM is set to drive breakthroughs in biological imaging and make significant contributions to the study of biomaterials and bioengineered structures within material science.

ScienceDirect — The development of wearable biosensors has revolutionized real-time health monitoring, enabling continuous and personalized insights into physiological states. Metal-organic frameworks (MOFs), with their unique properties including high surface area, tunability, and molecular selectivity, have emerged as promising materials for enhancing the sensitivity and specificity of wearable biosensors. This review explores recent advances in MOF-based wearable biosensors, highlighting their role in detecting key biomarkers for a range of health applications. This review discusses how MOFs improve analyte binding, signal transduction, and stability in physiological conditions, as well as the integration of these sensors with artificial intelligence (AI) and machine learning (ML) algorithms to enhance data processing and predictive analysis. Despite challenges such as ensuring biocompatibility, long-term stability, and scalability, wearable MOF biosensors represent a new frontier in personalized healthcare. Their potential to monitor multiple biomarkers simultaneously and provide continuous, accurate health assessments paves the way for future innovations in telemedicine and remote patient monitoring, ultimately contributing to improved healthcare outcomes and proactive disease management.

ScienceDirect — Detection of viruses, including coronavirus (SARS-CoV-2), via facile, fast, and optical methods is highly important to control pandemics. In this regard, optically-active nanomaterials and nanoparticles (NPs) are a wise choice due to their long-term stability, ease of functionalization, and modifications. In this work, a nanocomposite based on NiFe layered double hydroxide (LDH) and ZIF-67 metal-organic framework (MOF) was designed and synthesized, and decorated on the surface of the melt-blown mask. The developed nanocomposite has a fluorescence emission at 625 nm. The selectivity of the nanocomposite towards the SARS-CoV-2 spike protein S1 was increased by adding CuO NPs. The limit of detection (LOD) of 1.5 nM and 24.5 nM against SARS-CoV-2 spike protein S1 was recorded by NiFe LDH@ZIF-67@CuO nanocomposite, and NiFe LDH@ZIF-67@CuO decorated on the surface of melt-blown. Also, in the presence of potential competitors and other types of pathogens, including Influenza virus types A and B, Staphylococcus aureus bacteria, and even cations/macromolecules, the fluorescence intensity changes had more than 40 % difference.

ScienceDirect — Super-resolution microscopy has emerged as a groundbreaking technique in cell biology, enabling researchers to visualize proteins and their interactions with unprecedented spatial resolution. This perspective highlights the transformative impact of super-resolution microscopy on protein imaging, emphasizing its significance in understanding cellular architecture and function. This review article discusses various labeling strategies, including fluorescent proteins, organic dyes, and CRISPR/Cas9-mediated tagging, each with unique advantages and considerations for optimizing protein visualization. The applications of super-resolution microscopy span across mapping protein complexes, tracking dynamics in live cells, and investigating disease mechanisms in cancer, neurodegenerative disorders, and infectious diseases, thereby playing a crucial role in drug discovery and target validation. However, challenges such as resolution-speed trade-offs, photobleaching, and data processing complexities must be addressed to enhance its utility. This review article explores emerging trends and future directions, including the integration of super-resolution with cryo-electron microscopy, advances in live-cell imaging, and the incorporation of nanoparticles and quantum dots for improved imaging capabilities. Automation and machine learning are poised to revolutionize data analysis, facilitating the exploration of increasingly complex biological questions. In conclusion, super-resolution microscopy not only expands our understanding of protein dynamics and interactions but also holds promise for future applications in biomedical research, diagnostics, and therapeutics, ultimately enriching the field of systems biology.

Royal Society of Chemistry — Wearable microneedle (MN) patches have emerged as a transformative platform for biosensing, offering a minimally invasive and user-friendly approach to real-time health monitoring and disease diagnosis. Primarily designed to access interstitial fluid (ISF) through shallow skin penetration, MNs enable precise

ACS Publications — Sustainable Materials for Regenerative Applications. This book explores the intersection between sustainability and biomedical innovation, focusing on how environmentally friendly materials can revolutionize tissue engineering. It explores the latest developments in green biomaterials and their applications, including biopolymers, bioinspired scaffolds, and inorganic-organic hybrids. By examining the integration of eco-conscious design principles into regenerative medicine, this volume bridges the gap between sustainability goals and medical science. Traditional synthetic biomaterials often come with environmental drawbacks such as waste generation and reliance on petrochemicals.

Wiley Online Library — Neurodegenerative diseases, particularly Alzheimer's disease and Parkinson's disease, present formidable challenges in modern medicine due to their complex pathologies and the absence of curative treatments. Despite advances in symptomatic management, early diagnosis remains essential for mitigating disease progression and improving patient outcomes. Traditional diagnostic methods, such as MRI, PET, and cerebrospinal fluid biomarker analysis, are often inadequate for the early detection of these diseases. Emerging porous materials, including metal–organic frameworks (MOFs), covalent–organic frameworks (COFs), MXene, zeolites, and porous silicon, offer promising new approaches for the early diagnosis of neurodegenerative diseases. These materials, characterized by highly tunable physicochemical properties, have the potential to capture and concentrate disease-specific biomarkers such as amyloid-beta (Aβ), tau protein, and alpha-synuclein (α-Syn). The integration of these materials into advanced biosensors for real-time detection holds the promise of revolutionizing neurodiagnostic, enabling non-invasive, highly sensitive, and specific detection platforms. Furthermore, the incorporation of artificial intelligence (AI) and machine learning (ML) techniques into the analysis of sensor data enhances diagnostic accuracy and allows for more efficient interpretation of complex biomarker profiles. AI and ML can optimize feature selection, improve pattern recognition, and facilitate the prediction of disease progression, making them indispensable tools for personalized medicine. This review explores the potential of porous materials in neurodegenerative disease diagnostics, emphasizing their design, functionality, and the synergistic role of AI and ML in advancing clinical applications.

Wiley Online Library — Bacterial cellulose is a biodegradable and ecologically safe material that has the potential to convert mechanical vibrations into electrical energy. This review introduces green energy harvesting, a novel concept that harnesses natural processes to provide sustainable energy. A thorough overview of bacterial cellulose, covering its distinctive features, its biological origin, and its energy conversion process, is fully presented. The different materials and methods used to design and fabricate bacterial cellulose-based energy harvesters are explored. Moreover, the various applications and benefits of these devices in the context of renewable energy are examined. The current challenges and limitations of this emerging field are identified and the possible avenues for future research are suggested. The significance of adopting eco-friendly approaches in achieving a balance between human needs and environmental preservation is highlighted. By providing a comprehensive and critical assessment of bacterial cellulose as a green energy harvester, this review aims to motivate researchers, engineers, and policymakers to tap into the rhythmic potential of this natural material in building a more sustainable and resilient future.

ScienceDirect — Microfluidic technologies, which leverage the distinctive fluid properties at the nanoscale and microscale, are experiencing a surge in interest and advancements across various scientific fields. In particular, integrating nanotechnology with microfluidics has significantly enhanced the synthesis, production, and targeted delivery of biomedical compounds, especially through nanoparticles. Researchers are increasingly drawn to microfluidic devices due to their numerous advantages, which include high reproducibility, uniform structural characteristics, potential for cost reduction, and highly controllable fluid dynamics. The unique attributes of microfluidic systems enable drug delivery platforms to achieve stability, enhanced potency, and tailored release profiles, which are critical for effective therapeutic interventions. Despite the substantial progress made in the area of drug delivery utilizing microfluidics, there remain significant concerns regarding the quality and consistency of the materials produced. Among the various materials being explored, polysaccharides stand out as a vital component of the biopolymer family, attracting considerable attention for their favorable properties. These include biodegradability, widespread natural availability, non-toxic profiles, and low production costs. Such advantageous characteristics position polysaccharides as promising candidates for formulating drug delivery systems that can encapsulate and guard sensitive pharmaceutical agents while ensuring their controlled release at targeted sites within the body. Particularly noteworthy is alginate (AlgI), a polysaccharide derived from brown seaweed, which serves as a focal point in this discussion. This article specifically reviews the opportunities and challenges associated with AlgI in the context of microfluidic-assisted drug delivery. By systematically examining recent literature, the article aims to provide a comprehensive and current perspective on recent research and innovations in this domain. This includes exploring the design and formulation of high-performance drug delivery systems that utilize microfluidic techniques combined with stimuli-responsive features of AlgI, an area that has been relatively underexplored in existing studies.

The Lancet — Global health has steadily improved over the past 30 years as measured by age-standardised DALY rates. After taking into account population growth and ageing, the absolute number of DALYs has remained stable. Since 2010, the pace of decline in global age-standardised DALY rates has accelerated in age groups younger than 50 years compared with the 1990–2010 time period, with the greatest annualised rate of decline occurring in the 0–9-year age group. Six infectious diseases were among the top ten causes of DALYs in children younger than 10 years in 2019: lower respiratory infections (ranked second), diarrhoeal diseases (third), malaria (fifth), meningitis (sixth), whooping cough (ninth), and sexually transmitted infections (which, in this age group, is fully accounted for by congenital syphilis; ranked tenth). In adolescents aged 10–24 years, three injury causes were among the top causes of DALYs: road injuries (ranked first), self-harm (third), and interpersonal violence (fifth). Five of the causes that were in the top ten for ages 10–24 years were also in the top ten in the 25–49-year age group: road injuries (ranked first), HIV/AIDS (second), low back pain (fourth), headache disorders (fifth), and depressive disorders (sixth). In 2019, ischaemic heart disease and stroke were the top-ranked causes of DALYs in both the 50–74-year and 75-years-and-older age groups. Since 1990, there has been a marked shift towards a greater proportion of burden due to YLDs from non-communicable diseases and injuries. In 2019, there were 11 countries where non-communicable disease and injury YLDs constituted more than half of all disease burden. Decreases in age-standardised DALY rates have accelerated over the past decade in countries at the lower end of the SDI range, while improvements have started to stagnate or even reverse in countries with higher SDI.

JACC Specialty Journals — Cardiovascular diseases (CVDs), principally ischemic heart disease (IHD) and stroke, are the leading cause of global mortality and a major contributor to disability. This paper reviews the magnitude of total CVD burden, including 13 underlying causes of cardiovascular death and 9 related risk factors, using estimates from the Global Burden of Disease (GBD) Study 2019. GBD, an ongoing multinational collaboration to provide comparable and consistent estimates of population health over time, used all available population-level data sources on incidence, prevalence, case fatality, mortality, and health risks to produce estimates for 204 countries and territories from 1990 to 2019.

The Lancet — Rigorous analysis of levels and trends in exposure to leading risk factors and quantification of their effect on human health are important to identify where public health is making progress and in which cases current efforts are inadequate. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 provides a standardised and comprehensive assessment of the magnitude of risk factor exposure, relative risk, and attributable burden of disease.

ScienceDirect — The rapid diagnosis of pathogens is crucial in the early stages of treatment of diseases where the choice of the correct drug can be critical. Although conventional cell culture-based techniques have been widely utilized in clinical applications, newly introduced optical-based, microfluidic chips are becoming attractive. The advantages of the novel methods compared to the conventional techniques comprise more rapid diagnosis, lower consumption of patient sample and valuable reagents, easy application, and high reproducibility in the detection of pathogens. The miniaturized channels used in microfluidic systems simulate interactions between cells and reagents in microchannel structures, and evaluate the interactions between biological moieties to enable diagnosis of microorganisms. The overarching goal of this review is to provide a summary of the development of microfluidic biochips and to comprehensively discuss different applications of microfluidic biochips in the detection of pathogens. New types of microfluidic systems and novel techniques for viral pathogen detection (e.g. HIV, HVB, ZIKV) are covered. Next generation techniques relying on high sensitivity, specificity, lower consumption of precious reagents, suggest that rapid generation of results can be achieved via optical based detection of bacterial cells. The introduction of smartphones to replace microscope based observation has substantially improved cell detection, and allows facile data processing and transfer for presentation purposes.

ScienceDirect — Nanogels are three-dimensional nanoscale networks formed by physically or chemically cross-linking polymers. Nanogels have been explored as drug delivery systems due to their advantageous properties, such as biocompatibility, high stability, tunable particle size, drug loading capacity, and possible modification of the surface for active targeting by attaching ligands that recognize cognate receptors on the target cells or tissues. Nanogels can be designed to be stimulus responsive, and react to internal or external stimuli such as pH, temperature, light and redox, thus resulting in the controlled release of loaded drugs. This “smart” targeting ability prevents drug accumulation in non-target tissues and minimizes the side effects of the drug. This review aims to provide an introduction to nanogels, their preparation methods, and to discuss the design of various stimulus-responsive nanogels that are able to provide controlled drug release in response to particular stimuli.