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Developing the Computational Image Processing Method for Quantitative Analysis of Nanopore Structure Obtained from HS-AFM (AFMnanoQ)

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By Hoang Nguyen Verified, Hoàng Nguyên, Hoàng Nguyễn Verified, Kien Ngo, Ho Chi Minh, Ngoc Quoc Ly
| biorxiv.org
Abstract High-Speed Atomic Force Microscopy (HS-AFM) enables nanoscale imaging of biological structures with exceptional spatial (1 nm in the x-y plane; ~0.1 nm in the z-direction) and temporal resolution (~20 ms per frame). HS-AFM encodes three-dimensional (3D) information within a two-dimensional (2D) format, where the lateral dimensions (x, y) of structures correspond to spatial positioning in the image, while height (z) information is embedded in pixel intensity.
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