Niclas Skarne on Muck Rack

Niclas Skarne

As seen in: The BMJ
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Articles

EphA3 CAR T cells are effective against glioblastoma in preclinical models

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By Paulo Henrique Martins, Shane Horsefield, Niclas Skarne, Lea Lekieffre
| The BMJ Verified
Methods DNA sequence of the extracellular domain of EphA3 (P29320 amino acids 21–541) with a polypetide histidine tag was cloned into the mammalian expression vector pcDNA3.4 and the EphA3 protein was expressed in Expi293F cells. EphA3 protein was purified from the cell culture supernatant by a one-step purification by HisTrap FF Crude.
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