Nicole D. Barth

Peptide 7 enables selective imaging of GPR54 in human cells Having confirmed the agonist activity of peptide 7, we next assessed its application for fluorescence imaging of HEK293 cells that stably expressed hGPR54 equipped with an additional N-terminal hemagglutinin (HA) tag for dual color staining. First, we observed bright fluorescence labeling of transfected HEK293 cells after incubation with peptide 7 using fluorescence confocal microscopy (Figure 2a).

Results and Discussion We first designed chemo-cat NIR as a NIR fluorescent probe to image TAMs by exploiting a sequential activation mechanism, whereby the probe would 1) enter cells via CCR2-mediated endocytosis and 2) undergo fluorophore activation by reaction with intracellular cysteine cathepsins (Scheme 1). We envisioned that a molecular AND-gate design could lead to selective labeling of CCR2+ TAMs containing active cysteine cathepsins, but not any other cells found in the TME.

Abstract We describe Apotracker Red as a single fluorescent activatable probe for robust quantification of cancer cell death in fluorescence-based assays and in vivo using multiphoton intravital imaging in mouse models of breast cancer. Abstract The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models.