Nicole Simone Treichel

Abstract Metabolic dysfunction associated steatotic liver disease (MASLD) presents a growing global health problem with a range of manifestations, including steatosis, steatohepatitis, and cirrhosis. It is strongly associated with obesity, disease progression being promoted not only by hepatic leukocyte accumulation but also by inflammatory signals from adipose tissue and an altered gut microbiome.

Abstract Shallow metagenomics promises taxonomic and functional insights into samples at an affordable price.To determine the depth of sequencing required for specific analysis, benchmarking is required using defined communities. We used complex mixtures of DNA from cultured gut bacteria and analysed taxonomic composition, strain-level resolution, and functional profiles at nine sequencing depths (0.1-10.0 Gb).