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Nikolette McCombs on Muck Rack

Nikolette McCombs

As seen in: Bioprocess Online, Cell & Gene
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Articles

Enhancing Host Cell DNA Testing With Digital PCR: Efficiency Gains For Biopharmaceutical Manufacturing Original

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By Jamie Betker, David Abraham, Nikolette McCombs
| Bioprocess Online Verified
By Jamie Betker, David Abraham, Nikolette McCombs, and Jason Duex Residual host cell DNA remains a critical impurity to monitor in biopharmaceutical production, with increasing pressure to improve accuracy, speed, and consistency across workflows. In this collaborative study, KBI Biopharma and QIAGEN demonstrate how digital PCR can simplify residual DNA testing while maintaining robust analytical performance across CHO- and E. coli-derived bioprocesses.
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Oxidation of Pyrrole by Dehaloperoxidase-Hemoglobin: Chemoenzymatic Synthesis of Pyrrolin-2-Ones

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By Tatyana Smirnova, Nikolette McCombs, Reza Ghiladi
| Royal Society of Chemistry Verified
The use of oxidoreductases as biocatalysts in the syntheses of functionalized, monomeric pyrroles has been a challenge owing to, among a number of factors, undesired polypyrrole formation. Here, we have investigated the ability of dehaloperoxidase (DHP), the coelomic hemoglobin from the terebellid polychaete Amphitrite ornata, to catalyze the H O -dependent oxidation of pyrroles as a new class of substrate for this enzyme.
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