Nontombi Mbelle on Muck Rack

Nontombi Mbelle

As seen in: Nature, Wiley Online Library, medRxiv
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Articles

Plasmid-borne mcr-1 and Replicative Transposition of Episomal and Chromosomal blaNDM-1, blaOXA-69, and blaOXA-23 Carbapenemases in a Clinical Acinetobacter baumannii Isolate

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By Masego Mmatli, Nontombi Mbelle, John Osei Sekyere
| biorxiv.org
Abstract Background: A multidrug-resistant clinical A. baumannii isolate with resistance to most antibiotics was isolated from a patient at an intensive care unit. The genetic environment, transcriptome, mobile, and resistome were characterized. Method: The MicroScan system, disc diffusion, and broth microdilution were used to determine the resistance profile of the isolate. A multiplex PCR assay was also used to screen for carbapenemases and mcr-1 to -5 resistance genes.
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Evaluation of Six Commercial and Non-Commercial Colistin Resistance Diagnostics

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By Masego Mmatli, Lebogang Skosana, Nontombi Mbelle, John Osei Sekyere
| medRxiv
The authors have declared no competing interest. This work was funded by a grant from the National Health Laboratory Service (NHLS) given to Dr. John Osei Sekyere under grant number GRANT004 94807 and GRANT004 94808. I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.
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Genomic, Epigenomic, and Transcriptional Characterisation of Carbapenem and Colistin Resistance Mechanisms in Klebsiella pneumoniae and Enterobacter species.

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By Nontombi Mbelle, John Osei Sekyere, P Fourie, Masego Mmatli
| biorxiv.org
Abstract The emergence of colistin and carbapenem-resistant Klebsiella pneumoniae isolates presents a significant global health threat. This study investigates the resistance mechanisms in six K. pneumoniae and four Enterobacter sp. isolates lacking carbapenemases or mcr genes using genomics and transcriptomics.
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