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As seen in: Wiley Online Library, Clinical Chemistry
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Articles

Microsatellite Instability Detection in Cancer: A Multiplex qPCR Approach that Obviates the Need for Matching Normal Samples

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By NuProbe USA, David Yu, Yan Helen, Jinny Xuemeng
| Clinical Chemistry Verified
Microsatellite instability (MSI) indicates DNA mismatch repair deficiency in certain types of cancer, such as colorectal cancer. The current gold standard technique, PCR–capillary electrophoresis (CE), requires matching normal samples and specialized instrumentation. We developed VarTrace, a rapid and low-cost quantitative PCR (qPCR) assay, to evaluate MSI using solely the tumor sample DNA, obviating the requirement for matching normal samples.
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Enhanced Detection of Novel Low‐Frequency Gene Fusions via High‐Yield Ligation and Multiplexed Enrichment Sequencing

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By Yi Wu, Jinxiao Guo, Wenjun Li, NuProbe USA
| Wiley Online Library Verified
Conflict of interests There are patents pending on the BDA (patent number EP3146080B1) and mBDA (patent number WO2019164885A1) methods used in this work. There is also a patent describing the methods and compositions for sequencing and fusion detection using ligation tail adapters (No. P35027WO00) used in this work. P.S., W.L., and D.T. declare competing interests in the form of consulting for Nuprobe USA.
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