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Articles

Digitalized organoids: integrated pipeline for high-speed 3D analysis of organoid structures using multilevel segmentation and cellular topology - Nature Methods

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By Hui Ting Ong, Gianluca Grenci, Alessandro Furlan, Blake Johnson, Cora S. Thiel, OrgaRes Consortium, Victor Racine, Dimitri Mikec, Gaetan Galisot, Albert Zou Liu
| Nature Verified
Abstract Organoids replicate tissue architecture and function and offer a unique opportunity to explore the impact of external perturbations in vitro. However, conducting large-scale screening procedures to investigate the effects of various stresses on cellular morphology and topology in these systems poses important challenges, including limitations in high-resolution three-dimensional (3D) imaging and accessible 3D analysis platforms.
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