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Abstract The availability of certain amino acids regulates cell survival, proliferation, growth, differentiation, and other cellular functions. Sensing of amino acids that converges on the GATOR1 and GATOR2 complexes supports activation of mTORC1 during amino acid replete conditions. Whether amino acid-derived cues regulate additional pathways remains poorly understood.
Protein O-GlcNAcylation regulates CCP dynamics To examine how OGT and protein O-GlcNAcylation may regulate CCP formation and dynamics and thus CME, we used siRNA gene silencing. As expected, OGT silencing results in a robust reduction in both OGT expression as well as the levels of protein O-GlcNAc modification ( Figure 1 A).
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